A2172
 |  |  |
 | Immunofluorescence Formalin-fixed, paraffin-embedded human tongue tissue stained with Monoclonal Anti-α-Sarcomeric Actin, Clone No. 5C5 (Cat. No. A2172). |
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A2172
Sigma
Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse
clone 5C5, ascites fluid
| MDL number: | MFCD00164521 |
Description
| Immunogen | rabbit striated muscle actin. |
| Other Notes | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin. |
| Specificity | The antibody is specific for the single isoform of α-skeletal and α-cardiac muscle actins. |
Properties
| antibody form | ascites fluid |
| clone | 5C5 (monoclonal) |
| contains | 15 mM sodium azide |
| species reactivity | sheep, snake, human, rabbit, guinea pig, rat, frog, bovine |
| application(s) | immunoblotting: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle | |
| immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle | |
| indirect ELISA: suitable | |
| isotype | IgM |
| Gene Information | human ... ACTA1(58), ACTC1(70) |
| rat ... Acta1(29437), Actc1(29275) | |
| mouse ... Rwdd2a(69519) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Skalli, O., et al., Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas. Am. J. Pathol. 130, 515-531, (1988) Abstract |
| Henzen, C., et al., Differential role of p300 and CBP acetyltransferase during myogenesis: p300 acts upstream of MyoD and Myf5 EMBO J. 22, 5186-5196, (2003) Abstract | |
| Beggs, A.H., et al., Nemaline Myopathy with Minicores Caused by Mutation of the CFL2 Gene Encoding the Skeletal Muscle Actin–Binding Protein, Cofilin-2 Am. J. Hum. Genet. 80, 162-167, (2007) Abstract | |
| Lowe, D.A., et al., Skeletal muscle-specific ablation of cyto-actin does not exacerbate the mdx phenotype. PLoS ONE 3, e2419, (2008) Abstract |
